Archives of Medical Research
Volume 35, Issue 6 , Pages 511-516 , November 2004

Comparison of polymerase chain reaction and catalyzed signal amplification in situ hybridization methods for human papillomavirus detection in paraffin-embedded cervical preneoplastic and neoplastic lesions

  • Mirela Mirt Dabić

      Affiliations

    • Department of Pathology, University Hospital Center, Zagreb, Croatia
    • Corresponding Author InformationAddress for correspondence: Mirela Mirt Dabić, Department of Pathology, University Hospital Center, Zagreb. Tel: 01 4566 878. Fax: 01 4921 151.
    • ,
  • Ljiljana Hlupić

      Affiliations

    • Department of Pathology, University Hospital Center, Zagreb, Croatia
    • ,
  • Damir Babić

      Affiliations

    • Department of Pathology, University Hospital Center, Zagreb, Croatia
    • ,
  • Stanko Jukić

      Affiliations

    • Department of Pathology, School of Medicine, University of Zagreb, Zagreb, Croatia
    • ,
  • Sven Seiwerth

      Affiliations

    • Department of Pathology, School of Medicine, University of Zagreb, Zagreb, Croatia

Received 12 November 2003 ,Accepted 2 July 2004.

References 

  1. De Viliers EM. Human pathogenic papillomavirus types. Curr Top Microbiol Immunol. 1994;186:1–12
  2. Zur Hausen H, de Viliers EM. Human papillomaviruses. Annu Rev Microbiol. 1994;48:427–447
  3. Zur Hausen H. Molecular pathogenesis of cancer of the cervix and its causation by specific human papillomavirus types. Curr Top Microbiol Immunol. 1994;186:131–150
  4. Kalantari M, Karlsen F, Johansson B, Sigurjonsson T. Human papillomavirus findings in relation to cervical intraepithelial neoplasia grade. Hum Pathol. 1997;28:899–904
  5. Zehbe I, Rylander E, Edlund K, Wadell G, Wilander E. Detection of human papillomavirus in cervical intra-epithelial neoplasia, using in situ hybridization and various polymerase chain reaction techniques. Virchows Arch. 1996;428(3):151–157
  6. Kulasingam SL, Hughes JP, Kiviat NB, Mao C, Weiss NS, Kuypers JM, et al. Evaluation of human papillomavirus testing in primary screening for cervical abnormalities. JAMA. 2002;288:1749–1757
  7. Solomon D, Schiffman M, Tarone B, ALTS group . Comparison of three management strategies for patients with atypical squamous cells of undetermined siginificance (ASCUS): baseline results from a randomized trial. J Natl Cancer Inst. 2001;93:293–299
  8. Vince A, Ivanisevic M, Harni V, Skalko D, Jeren T. Molecular detection of human papillomavirus in women with minor grade cervical cytology abnormalities. J Clin Virol. 2001;20:91–94
  9. Baay MFD, Quint GV, Koudstaal J, Hollema H, Duk JM, Burger MPM, et al. Comprehensive study of several general and type specific primer pairs for detection of human papillomavirus DNA by PCR in paraffin-embedded cervical carcinoma. J Clin Microbiol. 1996;34:745–747
  10. Bettinger D, Bernard B, Riethmuller D, Greslin I, Gay C, Lab M, et al. Human papillomavirus detection by nonisotopic in situ hybridization, in situ hybridization with signal amplification and in situ polymerase chain reaction. Eur J Histochem. 1999;43:185–198
  11. Birner P, Bachtriary B, Dreier B, Schindl M, Joura EA, Breitenecker G, et al. Signal-amplified colorimetric in situ hybridization for assessment of human papillomavirus infection in cervical lesions. Mod Pathol. 2001;14:702–709
  12. Unger ER, Vernon SD, Lee DR, Miller DL, Reeves WC. Detection of human papillomavirus in archival tissues: comparison of in situ hybridization and polymerase chain reaction. J Histochem Cytochem. 1998;46:535–540
  13. Zehbe I, Rylander E, Edlund K, Wadell G, Wilander E. Detection of human papillomavirus in cervical intra-epithelial neoplasia, using in situ hybridization and various polymerase chain reaction techniques. Virchows Arch. 1996;428:151–157
  14. Birner P, Bachtiary B, Dreier B, Schindl M, Joura EA, Breitenecker G, et al. Signal-amplified colorimetric in situ hybridization for assessment of human papillomavirus infection in cervical lesions. Mod Pathol. 2001;14(7):702–709
  15. Richard RM. Historia natural de la neoplasia cervical intraepithelial. Clin Obstet Gynecol. 1967;20:747–783
  16. Saiki RK, Scharf S, Faloona F. Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985;230:1350–1354
  17. Manos MM, Ting Y, Wright DK, Lewis AJ, Broker TR, Wolinsky SM. The use of polymerase chain reaction amplification for the detection of genital human papillomaviruses. Cancer Cells. 1989;7:209–214
  18. Snijders PJF, van Den Brule AJC, Schrijnemakers HJF, Snow G, Meijer CJLM, Walboomers JMM. The use of general primers in the polymerase chain reaction permits the detection of a broad spectrum of human papillomavirus genotypes. J Gen Virol. 1990;71:173–181
  19. Tieben LM, ter Schegget J, Minnaar RP, Bouwes Bavinck JN, Berkhout RJ, Vermeer BJ. Detection of cutaneous and genital HPV types in clinical samples by PCR using consensus primers. J Virol Methods. 1993;42:265–279
  20. Soler C, Allibert P, Chardonnet Y, Cros P. Detection of human papillomavirus types 6, 11, 16 and 18 in mucosal and cutaneous lesions by the multiplex polymerase chain reaction. J Virol Methods. 1991;35:143–157
  21. Van der Brule AJC, Snijders JPF, Gordijn RLJ, Bleker OP, Meijer CJLM, Walboomers JMM. General primer-mediated polymerase chain reaction permits the detection of sequenced and still unsequenced HPV genotypes in cervical scrapes and carcinomas. Int J Cancer. 1990;45:644–649
  22. Karlsen F, Kalantari M, Jenkins A. Use of multiple PCR primer sets for optimal detection of human papilomavirus. J Clin Microbiol. 1996;34:2095–2100
  23. Kleter B, van Doorn LJ, ter Schegget J, Schrauwen L, van Krimpen C, Burger MP, et al. A novel short fragment PCR assay for highly sensitive broad spectrum detection of anogenital human papillomavirus. Am J Pathol. 1998;153:1731–1739
  24. Kulasingam SL, Hughes JP, Kiviat NB, Mao C, Wiess NS, Kuypers JM, et al. Evaluation of human papillomavirus testing in primary screening for cervical abnormalities. JAMA. 2002;288:1749–1757

PII: S0188-4409(04)00104-3

doi: 10.1016/j.arcmed.2004.07.004

Archives of Medical Research
Volume 35, Issue 6 , Pages 511-516 , November 2004

Article Tools

* Image Usage & Resolution