Archives of Medical Research
Volume 35, Issue 6 , Pages 480-483 , November 2004

DNA damage in peripheral lymphocytes of untreated breast cancer patients

  • Patricia Sánchez

      Affiliations

    • Oncological Research Unit
  • ,
  • Rubicelia Peñarroja

      Affiliations

    • Oncological Research Unit
  • ,
  • Francisco Gallegos

      Affiliations

    • Department of Clinical Oncology, Oncology Hospital, National Medical Center S-XXI, Instituto Mexicano del Seguro Social (IMSS), Mexico City, Mexico
  • ,
  • José Luis Bravo

      Affiliations

    • Institute de Ciencias de la Atmósfera, Universidad Nacional Autónoma de México (UNAM), Mexico City, Mexico
  • ,
  • Emilio Rojas

      Affiliations

    • Institute de Investigaciones Biomédicas, UNAM, Mexico City, Mexico
  • ,
  • Luis Benítez-Bribiesca

      Affiliations

    • Oncological Research Unit
    • Corresponding Author InformationAddress reprint requests to: Luis Benítez-Bribiesca, M.D., Oncological Research Unit, Oncology Hospital, National Medical Center S-XXI, IMSS, Av. Cuauhtémoc #330, Col. Doctores, 06725 México, D.F, México, Telefax: (+52) (55) 5578-674

Received 27 August 2004 ,Accepted 30 August 2004.

References 

  1. Cheng KC, Loeb LA. Genomic instability and tumor progression: mechanistic considerations. Adv Cancer Res. 1993;60:121–156
  2. De la Torre C, Pincheira JJ, López-Saez JF. Human syndromes with genomic instability and multiprotein machines that repair DNA double-strand breaks. Histopathology. 2003;18:225–243
  3. Maluf SW, Erdtmann B. Genomic instability in Down syndrome and Fanconi anemia assessed by micronucleus analysis and single-cell gel electrophoresis. Cancer Genet Cytogenet. 2001;124:71–75
  4. Dhillon VS, Bhasker R, Kler RS, Husain SA. Sister chromatid exchange (SCE) studies in breast cancer patients: a follow-up study. Cancer Genet Cytogenet. 1995;80:115–117
  5. Rothfub A, Schütz P, Bochum S, Volm T, Eberhardt E, Kreienberg R, et al. Induced micronucleus frequencies in peripheral lymphocytes as a screening test for carriers of a BRCAl mutation in breast cancer families. Cancer Res. 2000;60:390–394
  6. Ramesh KH, Bhargava MK. Cytogenetic damage in peripheral blood lymphocytes of cancer patients prior to radiotherapy. Cancer Genet Cytogenet. 1992;60:86–88
  7. Colleu-Durel S, Guitton N, Nourgalieva K, Léveque J, Danic B, Chenal C. Genomic instability and breast cancer. Oncol Rep. 2001;8:1001–1005
  8. Colleu-Durel S, Guitton N, Nourgalieva K, Legue F, Léeeveque J, Danic B, et al. Alkaline single-cell gel electrophoresis (comet assay): a simple technique to show genomic instability in sporadic breast cancer. Eur J Cancer. 2004;40:445–451
  9. Singh NP, McCoy MT, Tice RR, Schneider EL. A simple technique for quantitation of low levels of DNA damage in individual cells. Exp Cell Res. 1988;175:184–191
  10. Olive PL, Banáth JP, Durand RE. Heterogeneity in radiation-induced DNA damage and repair in tumor and normal cells measured using the “comet” assay. Rad Res. 1990;122:86–94
  11. Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H, et al. Single cell/gel comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen. 2000;35:206–221
  12. Kopjar N, Garaj-Vrhovac V, Milas I. Assessment of chemotherapy-induced DNA damage in peripheral blood leukocytes of cancer patients using the alkaline comet assay. Teratog Carcinog Mutag. 2002;22:13–30
  13. Jackson SP. Sensing and repairing DNA double-strand breaks. Carcinogenesis. 2002;23:687–696
  14. Boyum A. Separation of white blood cells. Nature. 1964;204:793
  15. Olive PL, Wlodek D, Banath JP. DNA double-strand breaks measured in individual cells subjected to gel electrophoresis. Cancer Res. 1991;51:4671–4676
  16. Banáth JP, Fushiki M, Olive PL. Rejoining of DNA single- and double strand breaks in human white blood cells exposed to ionizing radiation. Int J Radiat Biol. 1998;73:649–660
  17. Benítez-Bribiesca L, Sánchez P, Toledo J, Peñarroja R, Flores M, Sosa J. Differential staining of DNA strand breaks in dried comet. J Histochem Cytochem. 2001;49:921–922
  18. Vaghef H, Nygren P, Edling C, Bergh J, Hellman B. Alkaline single-cell gel electrophoresis and human biomonitoring for genotoxicity: a pilot study on breast cancer patients undergoing chemotherapy including cyclophosphamide. Mutat Res. 1997;395:127–138
  19. Nascimento PA, da Silva MA, Oliveira EM, Suzuki MF, Okazak K. Evaluation of radioinduced damage and repair capacity in blood lymphocytes of breast cancer patients. Braz J Med Biol Res. 2001;34:165–176
  20. Lankinen M, Vilpo LM, Vilpo JA. UV and γ-irradiation-induced DNA single-strand breaks and their repair in human blood granulocytes and lymphocytes. Mutat Res. 1996;352:31–38
  21. Jaloszynski P, Kujawski M, Czub-Swierczek M, Markowska J, Szyfter K. Bleomycin-induced DNA damage and its removal in lymphocytes of breast cancer patients studied by comet assay. Mutat Res. 1997;385:223–233
  22. Smith T, Miller M, Lohman K, Case DL, Hu J. DNA damage and breast cancer risk. Carcinogenesis. 2003;24:883–889

PII: S0188-4409(04)00139-0

doi: 10.1016/j.arcmed.2004.11.008

Archives of Medical Research
Volume 35, Issue 6 , Pages 480-483 , November 2004