Archives of Medical Research
Volume 37, Issue 6 , Pages 696-699 , August 2006

Catalytic Inactivation of Human Phospholipase D2 by a Naturally Occurring Gly901Asp Mutation

  • Yoshiji Yamada

      Affiliations

    • Department of Human Functional Genomics, Life Science Research Center, Mie University, Tsu, Japan
    • Corresponding Author InformationAddress reprint requests to: Yoshiji Yamada, MD, PhD, FAHA, Department of Human Functional Genomics, Life Science Research Center, Mie University, 1577 Kurima-machiya, Tsu, Mie 514-8507, Japan
    • ,
  • Yoshiko Banno

      Affiliations

    • Department of Cell Signaling, Gifu University Graduate School of Medicine, Gifu, Japan
    • ,
  • Hitoshi Yoshida

      Affiliations

    • Molecular Oncology Division, National Cancer Center Research Institute, Tokyo, Japan
    • ,
  • Ryosuke Kikuchi

      Affiliations

    • Nagoya University School of Health Sciences, Nagoya, Japan
    • ,
  • Yukihiro Akao

      Affiliations

    • Gifu International Institute of Biotechnology, Kakamigahara, Japan
    • ,
  • Takashi Murate

      Affiliations

    • Nagoya University School of Health Sciences, Nagoya, Japan
    • ,
  • Yoshinori Nozawa

      Affiliations

    • Gifu International Institute of Biotechnology, Kakamigahara, Japan

Received 1 September 2005 ,Accepted 4 January 2006.

References 

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  10. Gottlin EB, Rudolph AE, Zhao Y, Mattbews HR, Dixon JE. Catalytic mechanism of the phospholipase D superfamily proceeds via a covalent phosphohistidine intermediate. Proc Natl Acad Sci USA. 1998;95:9202–9207
  11. Xie Z, Ho W-T, Exton J. Association of N- and C-terminal domains of phospholipase D is required for catalytic activity. J Biol Chem. 1998;273:34679–34682
  12. Sung T-C, Altshuller YM, Morris AJ, Frohman MA. Molecular analysis of mammalian phospholipase D2. J Biol Chem. 1999;274:494–502
  13. Liu M-Y, Gutowski S, Sternweis PC. The C terminus of mammalian phospholipase D is required for catalytic activity. J Biol Chem. 2001;276:5556–5562
  14. Yamada Y, Hamajima N, Kato T, Iwata H, Yamamura Y, Shinoda M, et al. Association of a polymorphism of the phospholipase D2 gene with the prevalence of colorectal cancer. J Mol Med. 2003;81:126–131
  15. Xie Z, Ho W-T, Exton JH. Conserved amino acids at the C-terminus of rat phospholipase D1 are essential for enzymatic activity. Eur J Biochem. 2000;267:7138–7146
  16. Banno Y, Fujita H, Ono Y, Nakashima S, Ito Y, Kuzumaki N, et al. Differential phospholipase D activation by bradykinin and sphingosine 1-phosphate in NIH3T3 fibroblasts overexpressing gelsolin. J Biol Chem. 1999;274:27385–27391
  17. Ohguchi K, Banno Y, Nakashima S, Nozawa Y. Regulation of membrane-bound phospholipase D by protein kinase C in HL60 cells. Synergistic action of small GTP-binding protein RhoA. J Biol Chem. 1996;271:4366–4372

PII: S0188-4409(06)00083-X

doi: 10.1016/j.arcmed.2006.01.006

Archives of Medical Research
Volume 37, Issue 6 , Pages 696-699 , August 2006

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