Archives of Medical Research
Volume 37, Issue 6 , Pages 723-729, August 2006

In Vitro Antiproliferative Effects of Neuroleptics, Antimycotics and Antibiotics on the Human Pathogens Acanthamoeba polyphaga and Naegleria fowleri

  • Raúl N. Ondarza

      Affiliations

    • Center for Research on Infectious Diseases, National Institute of Public Health, Cuernavaca, Morelos, Mexico
    • Department of Biochemistry, Faculty of Medicine, National Autonomous University of Mexico, Mexico City, Mexico
    • Center for Marine Biotechnology and Biomedicine, Scripps Institution of Oceanography, University of California–San Diego (UCSD), La Jolla, California
    • Corresponding Author InformationAddress reprint requests to: Raúl N. Ondarza, Olivar de los Padres 941, 0178 México, D.F., México.
    • ,
  • Angelica Iturbe

      Affiliations

    • Center for Research on Infectious Diseases, National Institute of Public Health, Cuernavaca, Morelos, Mexico
    • ,
  • Eva Hernández

      Affiliations

    • Center for Research on Infectious Diseases, National Institute of Public Health, Cuernavaca, Morelos, Mexico

Received 25 November 2005; accepted 2 February 2006.

(ARCMED-D-05-00488)

Background

Using reproducible conditions in vitro, the aim of this study was to obtain a comparative evaluation of the efficacies of several tricyclic neuroleptics, antimycotics and antibiotics with antiproliferative activities against Acanthamoeba polyphaga and Naegleria fowleri trophozoites.

Methods

We used reproducible conditions in vitro to obtain results.

Results

The most effective drugs against N. fowleri expressed as (IC50) were as follows: the antimycotics ketoconazole and amphotericin B, followed by trifluoperazine, mepacrine, chlorpromazine, miconazole, and metronidazole. The least effectives were rifampicin and pentamidine. The most potent growth inhibitors (MIC100) against N. fowleri were the antimycotics amphotericin B and ketoconazole and the neuroleptic trifluoperazine. It was clear that there are major differences between the two amebas in their susceptibility to some of the drugs.

Conclusions

The drugs with the minimal inhibitory concentration (MIC) values could be considered alone or in combination as potential anti-amebic agents for the treatment of the diseases produced by these amebas.

Key Words: Acanthamoeba polyphaga, Naegleria fowleri, Neuroleptics, Antimycotics, Antibiotics, Antiparasitic agents

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PII: S0188-4409(06)00096-8

doi:10.1016/j.arcmed.2006.02.007

Refers to erratum:

  • Erratum

    Archives of Medical Research November 2006 (Vol. 37, Issue 8, Page 1038)

Archives of Medical Research
Volume 37, Issue 6 , Pages 723-729, August 2006

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