Tumoral Cell mtDNA ∼8.9 kb Deletion Is More Common than Other Deletions in Gastric Cancer

Kamalidehghan, Behnam; Houshmand, Massoud; Panahi, Mehdi Shafa Shariat; Abbaszadegan, Mohammad Reza; Ismail, Patimah; Shiroudi, Mahnaz Bagher

doi:10.1016/j.arcmed.2006.03.007

« Previous Next »Archives of Medical Research
Volume 37, Issue 7 , Pages 848-853, October 2006

Tumoral Cell mtDNA ∼8.9 kb Deletion Is More Common than Other Deletions in Gastric Cancer

Behnam Kamalidehghan
- Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
- Department of Biomedical Sciences, Putra University Malaysia, Selangor, Malaysia
Massoud Houshmand
- Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
- Address reprint requests to: Massoud Houshmand, Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd. Tehran-Karaj Highway, 17th Km., P.O. Box 14155-6343, Tehran, Iran
Mehdi Shafa Shariat Panahi
- Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
Mohammad Reza Abbaszadegan
- Department of Human Genetics, Bu-Ali Research Institute, Mashhad, Iran
Patimah Ismail
- Department of Biomedical Sciences, Putra University Malaysia, Selangor, Malaysia
Mahnaz Bagher Shiroudi
- Department of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran

Received 17 December 2005; accepted 3 March 2006.

(ARCMED-D-05-00514)

Background

The aim of the study was to clarify the role of deletion of mitochondrial DNA (mtDNA) in gastric carcinogenesis and to determine prevalence of mitochondrial deletions in different regions of tumoral tissue in comparison with adjacent non-tumoral tissue in gastric cancer.

Methods

In order to investigate whether a high incidence of mutations exists in mtDNA of gastric cancer tissues, we screened five regions of the mitochondrial genome by PCR amplification, Southern blot and DNA sequence analysis.

Results

Of 71 cancer patients, the ∼8.9 kb deletion was detected among different deletions in 9 cases (12.67%) of the tumoral tissues and 1 case (1.40%) in non-tumoral tissues that were adjacent to the tumors. Level of the 8.9 kb deletion has been found to be more than other deletions in tumoral tissues.

Conclusions

The ∼8.9 kb deletion has an obvious correlation with age and histological type. These data suggest that the ∼8.9 kb deletion in mtDNA may play an important role in gastric carcinogenesis.

Key Words: Gastric cancer, ∼8.9 kb deletion, Tumoral cell mtDNA