Tumoral Cell mtDNA ∼8.9 kb Deletion Is More Common than Other Deletions in Gastric Cancer
Received 17 December 2005; accepted 3 March 2006.
Background
The aim of the study was to clarify the role of deletion of mitochondrial DNA (mtDNA) in gastric carcinogenesis and to determine prevalence of mitochondrial deletions in different regions of tumoral tissue in comparison with adjacent non-tumoral tissue in gastric cancer.
Methods
In order to investigate whether a high incidence of mutations exists in mtDNA of gastric cancer tissues, we screened five regions of the mitochondrial genome by PCR amplification, Southern blot and DNA sequence analysis.
Results
Of 71 cancer patients, the ∼8.9 kb deletion was detected among different deletions in 9 cases (12.67%) of the tumoral tissues and 1 case (1.40%) in non-tumoral tissues that were adjacent to the tumors. Level of the 8.9 kb deletion has been found to be more than other deletions in tumoral tissues.
Conclusions
The ∼8.9 kb deletion has an obvious correlation with age and histological type. These data suggest that the ∼8.9 kb deletion in mtDNA may play an important role in gastric carcinogenesis.
aDepartment of Medical Genetics, National Institute for Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran
bDepartment of Biomedical Sciences, Putra University Malaysia, Selangor, Malaysia
cDepartment of Human Genetics, Bu-Ali Research Institute, Mashhad, Iran
Address reprint requests to: Massoud Houshmand, Department of Medical Genetics, National Institute of Genetic Engineering and Biotechnology (NIGEB), Pajoohesh Blvd. Tehran-Karaj Highway, 17th Km., P.O. Box 14155-6343, Tehran, Iran
ABSTRACT