Archives of Medical Research
Volume 40, Issue 4 , Pages 249-255, May 2009

Role of the Spleen in Cyclophosphamide-induced Hematosuppression and Extramedullary Hematopoiesis in Mice

  • Yuli Wang

      Affiliations

    • The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School of Harbin Medical University, Harbin, China
    • These authors contributed equally to this work.
    • ,
  • Qinggang Meng

      Affiliations

    • Department of Orthopedic Surgery, The Fourth Affiliated Hospital of Harbin Medical University, Harbin, China
    • These authors contributed equally to this work.
    • ,
  • Haiquan Qiao

      Affiliations

    • The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School of Harbin Medical University, Harbin, China
    • Corresponding Author InformationAddress reprint requests to: Haiquan Qiao, The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School of Harbin Medical University, Harbin 150001 China
    • ,
  • Hongchi Jiang

      Affiliations

    • The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School of Harbin Medical University, Harbin, China
    • ,
  • Xueying Sun

      Affiliations

    • The Hepatosplenic Surgery Center, Department of General Surgery, The First Clinical Medical School of Harbin Medical University, Harbin, China

Received 4 September 2008; accepted 25 March 2009. published online 05 June 2009.

(ARCMED-D-08-00398)

Background and Aims

Extramedullary hematopoiesis (EMH) is induced in spleens due to various diseases. The aim of this study is to investigate the role of spleen in cyclophosphamide (CTX)-induced hematosuppression and EMH in mice.

Methods

Balb/c mice were IP injected with 300 mg/kg CTX 2 weeks after splenectomy or sham operation and randomly sacrificed 1, 3, 7, 14, and 21 days after injection. Blood samples were collected, and spleens were weighed, histologically analyzed, and then used for flow cytometry.

Results

There were significant differences in white blood count, red blood count, platelet numbers and hemoglobin concentration between the splenectomized and sham-operated mice after CTX injection. The cellularity of the spleen was reduced 3 days following CTX treatment but then rose 7 days after CTX treatment. The numbers of colony-forming units in the spleen reached a peak 7 days after CTX injection, then declined. Flow cytometry demonstrated the percentage of CD34+ and CD117+ cells in the spleen increased 7 days after CTX injection, indicating the hematopoietic stem and progenitor cells in the spleen.

Conclusions

The study indicates that EMH occurs as a compensatory reaction to CTX-induced hematosuppression in the murine spleen, implying that conservation of the spleen may promote the recovery of cancer patients from chemotherapy-induced hematosuppression.

Key Words: Spleen, Extramedullary hematopoiesis, Cyclophosphamide, Hematosuppression

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PII: S0188-4409(09)00050-2

doi:10.1016/j.arcmed.2009.04.003

Refers to erratum:

  • Erratum

    Archives of Medical Research January 2010 (Vol. 41, Issue 1, Page 66)

Archives of Medical Research
Volume 40, Issue 4 , Pages 249-255, May 2009

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