Endothelial Nitric Oxide Synthase Gene Polymorphisms (894G > T and −786T > C) and Risk of Coronary Artery Disease in a Saudi Population
Received 4 October 2009; accepted 6 January 2010. published online 29 March 2010.
Background and Aims
Endothelial nitric oxide synthase gene polymorphisms, either independently or through gene environmental interactions, are associated with cardiovascular diseases in multiple ethnic populations. However, no information is available with regard to such associations in a Saudi population despite a high incidence of cardiovascular abnormalities. We studied the associations of 894G>T and −786T>C polymorphisms of endothelial nitric oxide synthase gene with coronary artery disease in Saudi population.
Methods
Variants 894G>T and −786T>C were studied in 142 coronary artery disease patients and 145 normal controls by PCR-restriction fragment length polymorphism analysis and allele specific PCR, respectively.
Results
Carriers of GT and TT genotypes of 894G>T polymorphism were significantly high (p <0.0001) in patients (47.2 and 7%, respectively) than in controls (27.6 and 4.8%, respectively). Likewise, carriers for TC and CC genotypes of −786T>C polymorphism were significantly high (p <0.001) in patients (50 and 32% respectively) than in controls (34.5 and 22.5% respectively). Both 894G>T [OR (95% CI); 4.39 (1.69–11.42)] and −786T>C [OR (95% CI); 2.74 (1.02–7.32]) variants were independently associated with the disease status. Genotype distributions of 894G>T and −786T>C polymorphisms in the diseased and control populations matched with those found in Caucasian populations.
Conclusion
This study, for the first time, suggests an independent association of 894G>T and −786T>C polymorphisms of endothelial nitric oxide synthase gene with coronary artery disease in a Saudi population.
aDepartment of Clinical Pharmacy, College of Pharmacy, King Saud University, Riyadh, Saudi Arabia
bDepartment of Biochemistry, College of Science, King Saud University, Riyadh, Saudi Arabia
Address reprint requests to: Khalid M. Alkharfy, Pharm.D., Ph.D., Assistant Professor, Department of Clinical Pharmacy, College of Pharmacy, King Saud University, PO Box 2457, Riyadh 11451, Saudi Arabia; Phone: 096614677494; FAX: 096614677480
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