Archives of Medical Research
Volume 41, Issue 2 , Pages 119-124 , February 2010

Detection of Fusion Genes in Formalin-fixed Paraffin-embedded Tissue Sections of Rhabdomyosarcoma by RT-PCR and Fluorescence In Situ Hybridization in Mexican Patients

  • Pilar Eguía-Aguilar

      Affiliations

    • Departamento de Patología, Hospital Infantil de México Federico Gómez, México, D.F., México
    • ,
  • Verónica Ponce-Castañeda

      Affiliations

    • Unidad de Investigación en Enfermedades Infecciosas, Hospital de Pediatría. Centro Médico Nacional Siglo XXI, IMSS, México, D.F., México
    • ,
  • Nayelli Nájera-García

      Affiliations

    • Servicio de Genética, Hospital General de México, Facultad de Medicina, UNAM, México, D.F., México
    • ,
  • Karem Nieto-Martínez

      Affiliations

    • Servicio de Genética, Hospital General de México, Facultad de Medicina, UNAM, México, D.F., México
    • ,
  • Susana Kofman-Alfaro

      Affiliations

    • Servicio de Genética, Hospital General de México, Facultad de Medicina, UNAM, México, D.F., México
    • ,
  • Stanislaw Sadowinski-Pine

      Affiliations

    • Departamento de Patología, Hospital Infantil de México Federico Gómez, México, D.F., México
    • ,
  • Pedro Valencia-Mayoral

      Affiliations

    • Departamento de Patología, Hospital Infantil de México Federico Gómez, México, D.F., México
    • ,
  • Francisco Arenas-Huertero

      Affiliations

    • Departamento de Patología, Hospital Infantil de México Federico Gómez, México, D.F., México
    • ,
  • Mario Perezpeña-Diazconti

      Affiliations

    • Departamento de Patología, Hospital Infantil de México Federico Gómez, México, D.F., México
    • Corresponding Author InformationAddress reprint requests to: Mario Pérezpeña-Díazconti, Departamento de Patología, Hospital Infantil de México Federico Gómez, Dr. Márquez 162, Col. Doctores, Del. Cuauhtémoc, C.P. 06720, México, D.F., México; Phone: (+52) (55) 5228-9917, ext. 1394; FAX: (+52) (55) 5761-8001

Received 30 October 2009 ,Accepted 25 January 2010.

References 

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  2. Pappo AS. Rhabdomyosarcomas and other soft tissue sarcomas of childhood. Curr Opin Oncol. 1995;7:361–366
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  8. Barr FG, Qualman SJ, Macris M. Genetic heterogeneity in the alveolar rhabdomyosarcoma subset without typical gene fusions. Cancer Res. 2002;62:4704–4710
  9. Chen BF, Chen ML, Liang DC, et al. Detection of PAX3- FKHR and PAX7- FKHR fusion transcripts in rhabdomyosarcoma by reverse transcriptase-polymerase chain reaction using paraffin-embedded tissue. Zhonghua Yi Xue Za Zhi (Taipei). 1999;62:86–91
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  18. Biegel JA, Nycum LM, Valentine V, et al. Detection of the t(2;13)(q35;q14) and PAX3- FKHR fusion in alveolar rhabdomyosarcoma by fluorescence in situ hybridization. Genes Chromosomes Cancer. 1995;12:186–192
  19. Mehra S, de la Roza G, Tull J, et al. Detection of FOXO1 (FKHR) gene break-apart by fluorescence in situ hybridization in formalin-fixed, paraffin-embedded alveolar rhabdomyosarcoma and its clinicopathologic correlation. Diagn Mol Pathol. 2008;17:14–20
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PII: S0188-4409(10)00012-3

doi: 10.1016/j.arcmed.2010.02.005

Archives of Medical Research
Volume 41, Issue 2 , Pages 119-124 , February 2010

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