Comparison of Bioartificial Pancreas Performance in the Bone Marrow Cavity and Intramuscular Space

Yang, Kai-Chiang; Wu, Chang-Chin; Qi, Zhi; Chen, Jung-Chih; Sumi, Shoichiro; Lin, Feng-Huei

doi:10.1016/j.arcmed.2010.03.002

« Previous Next »Archives of Medical Research
Volume 41, Issue 3 , Pages 151-153, April 2010

Comparison of Bioartificial Pancreas Performance in the Bone Marrow Cavity and Intramuscular Space

Kai-Chiang Yang
- Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
Chang-Chin Wu
- Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
- Department of Orthopedics, En Chu Kong Hospital, Taipei County, Taiwan
Zhi Qi
- Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- Department of Histology and Embryology, Nankai University School of Medicine, Nankai University, Tianjin, China
Jung-Chih Chen
- Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
- National Science Council, Taipei, Taiwan
Shoichiro Sumi
- Department of Organ Reconstruction, Institute for Frontier Medical Sciences, Kyoto University, Kyoto, Japan
Feng-Huei Lin
- Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, Taipei, Taiwan
- Division of Medical Engineering, National Health Research Institute, Miaoli County, Taiwan
- Address reprint requests to: Feng-Huei Lin, PhD, Prof., Institute of Biomedical Engineering, College of Medicine and College of Engineering, National Taiwan University, No. 1, Jen Ai Rd., Sec. 1, Taipei, 10051, Taiwan; Phone: (+886) 2-2312-3456 ex: 81456; FAX: (+886) 2-2394-0049

Received 13 November 2009; accepted 8 February 2010.

(ARCMED-D-09-00557)

Background and Aims

Bone marrow with a widely distributed and well-vascularized microenvironment that is capable of sustaining grafts is a potential site for islet transplantation. The femur bone marrow cavity offers sufficient space that may also receive the implantation of bioartificial pancreas (BAP).

Methods

Mouse insulinoma cells encapsulating in agarose gel were further enclosed in a calcium phosphate cement chamber to create a BAP. BAPs implanted into the femur bone marrow cavity of diabetics were compared with those implanted in the intramuscular space. Blood glucose level and C-peptide were determined perioperatively.

Results

The blood glucose level of the diabetics receiving BAPs in the intramuscular space decreased from 413 ± 24 to 285 ± 47 mg/dL at 1 day post-surgery. However, the blood glucose level returned to 398 ± 35 mg/dL with undetectable serum C-peptide at 2 weeks postoperatively that reveals implant failure. The blood glucose level of diabetics receiving BAPs into the femur bone marrow cavity decreased from 422 ± 32 to 247 ± 52 mg/dL and maintained in the range of 288 ± 47 mg/dL during the experimental period with an increase in C-peptide level from 6.1 ± 2.8 to 104.7 ± 16.4 pmol/L.

Conclusions

This preliminary study indicates that the effectiveness of BAPs transplanted into the femur bone marrow cavity is superior to that implanted in the intramuscular space, which reveals the bone marrow may be a potential receptor site for the BAP transplantation.

Key Words: Type 1 diabetes, Bioartificial pancreas, Bone marrow cavity, Intramuscular space