Apolipoprotein, C-Reactive Protein and Oxidative Stress Parameters in Dyslipidemic Type 2 Diabetic Patients Treated or Not with Simvastatin

Manfredini, Vanusa; Biancini, Giovana Brondani; Vanzin, Camila Simioni; Dal Vesco, Anna Maria Ribeiro; Wayhs, Carlos Alberto Yasin; Peralba, Maria do Carmo Ruaro; Vargas, Carmen Regla

doi:10.1016/j.arcmed.2010.03.006

« Previous Next »Archives of Medical Research
Volume 41, Issue 2 , Pages 104-109, February 2010

Apolipoprotein, C-Reactive Protein and Oxidative Stress Parameters in Dyslipidemic Type 2 Diabetic Patients Treated or Not with Simvastatin

Vanusa Manfredini
- Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul, Brazil
Giovana Brondani Biancini
- Serviço de Genética Médica, Laboratório de Análise de Metabólitos (LAM), Hospital de Clínicas de Porto Alegre, Brazil
Camila Simioni Vanzin
- Serviço de Genética Médica, Laboratório de Análise de Metabólitos (LAM), Hospital de Clínicas de Porto Alegre, Brazil
Anna Maria Ribeiro Dal Vesco
- Serviço de Genética Médica, Laboratório de Análise de Metabólitos (LAM), Hospital de Clínicas de Porto Alegre, Brazil
Carlos Alberto Yasin Wayhs
- Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul, Brazil
Maria do Carmo Ruaro Peralba
- Instituto de Química Inorgânica da Universidade Federal do Rio Grande do Sul (UFRGS), Brazil
Carmen Regla Vargas
- Programa de Pós-Graduação em Ciências Farmacêuticas da Universidade Federal do Rio Grande do Sul, Brazil
- Serviço de Genética Médica, Laboratório de Análise de Metabólitos (LAM), Hospital de Clínicas de Porto Alegre, Brazil
- Programa de Pós-Graduação em Ciências Biológicas Bioquímica, Brazil
- Addres reprint requests to: Carmen Regla Vargas, Serviço de Genética Médica, Hospital de Clínicas de Porto Alegre, R: Ramiro Barcelos, 2350, Bairro: Bom Fim, Porto Alegre RS, Brazil, 90035-003; Phone: (+55) (51) 3359 8011

Received 15 September 2009; accepted 13 February 2010.

(ARCMED-D-09-00435)

Background and Aims

Oxidative stress is considered an important factor in the development of diabetic complications that causes a variety of changes such as oxidative modification of membrane lipids, nucleic acids and cellular proteins. Dyslipidemia is frequently associated with diabetes and cardiovascular disease. In this context, the objective of this study was to evaluate oxidative modifications of plasma proteins and lipids in non dyslipidemic type 2 diabetic (T2D) patients, in dyslipidemic T2D patients treated or not with simvastatin and in healthy subjects to investigate whether treatment with low doses of simvastatin plays a protective role on the lipid and protein oxidative damage in these patients.

Methods

We determined oxidative damage of plasma proteins by carbonyl assay and total thiol group determination. We also characterized the membrane damage in terms of lipid peroxidation by measuring malonaldehyde (MDA) in nondyslipidemic T2D patients, dyslipidemic T2D patients treated with simvastatin (20 mg/day), dyslipidemic T2D patients not treated with simvastatin and in healthy age-matched control subjects.

Results

Our results showed that dyslipidemic T2D patients not treated with simvastatin had significantly higher plasma protein carbonyl groups and MDA when compared to dyslipidemic T2D patients treated with simvastatin and control group. Thiol concentrations from dyslipidemic T2D patients not treated with simvastatin were significantly lower than treated patients and controls. It was verified that the thiols groups were inversely correlated with apolipoprotein B and positively correlated with apolipoprotein A–I.

Conclusions

These results demonstrated that treatment with low doses of simvastatin can minimize the protein and lipid oxidative damage in dyslipidemic T2D patients.

Key Words: Dyslipidemic type 2 diabetes, Carbonyl, Thiol group, Malondialdehyde, Simvastatin