C677T Polymorphism of the 5,10 MTHFR Gene in Young Mexican Subjects with ST-Elevation Myocardial Infarction
Received 15 January 2010; accepted 13 April 2010.
Background and Aims
The C677T polymorphism of 5,10 methylenetetrahydrofolate reductase (MTHFR) gene has been associated with hypertension and coronary artery disease in several populations worldwide, but results are still controversial. The aim of this study was to examine the possible association of C677T polymorphism with ST-elevation myocardial infarction (STEMI) in young Mexican subjects.
Methods
In a case-control study, 167 unrelated patients ≤45 years of age with diagnosis of STEMI who were admitted to a cardiovascular intense care unit and 167 unrelated controls subjects matched by age and gender were recruited from January 2006 and June 2009. The C677T polymorphism was determined in all participants by a polymerase chain reaction-restriction fragment length polymorphism assay (PCR-RFLP).
Results
There was no significant difference in the genotype distribution between groups (p = 0.69) or allele frequency (p = 0.40). There were independent factors for STEMI: smoking (OR 4.9, 95% CI 3.0–8.1, p = 0.001), hypertension (OR 1.8, 95% CI 1.0–3.3, p = 0.03), family history of atherothrombotic disease (OR 2.3, 95% CI 2.0–4.6, p = 0.02), and dyslipidemia (OR 3.2, 95% CI 1.8–5.6, p <0.001). Diabetes mellitus did not represent an independent risk factor for STEMI (OR 1.2, 95% CI 0.2–2.2, p = 0.82).
Conclusions
The TT genotype from the C677T of 5,10 MTHFR gene is not an independent risk factor for STEMI in the Mexican population. However, more studies are needed to determine the possible “protective effect” of the C677T polymorphism in our population.
aUnidad de Investigación Médica en Trombosis, Hemostasia y Aterogénesis, H.G.R. No. 1, Dr. Carlos MacGregor Sánchez Navarro, Instituto Mexicano del Seguro Social, México, D.F., México
bEscuela de Ciencias Biológicas, Instituto Politécnico Nacional, México, D.F., México
cUnidad de Investigación Médica en Medicina Reproductiva UMAE, Hospital de Ginecología y Obstetricia No. 4, Luis Castelazo Ayala, Instituto Mexicano del Seguro Social, México, D.F., México
dHospital de Cardiología, Centro Médico Nacional Siglo XXI, Instituto Mexicano del Seguro Social, México, D.F., México
Address reprint requests to: Irma Isordia-Salas, M.D. PhD, Instituto Mexicano del Seguro Social, Thrombosis Research, Apartado Postal B32, Coahuila No. 5, Mexico, D.F. 06703, Mexico; Phone: (+52) (55) 5639-5822 ext. 20883; FAX: 55 56826051
ABSTRACT