Anti-oxidant Effects of Resveratrol on Mice with DSS-induced Ulcerative Colitis

Yao, Jun; Wang, Jian-Yao; Liu, Lei; Li, Ying-Xue; Xun, An-Ying; Zeng, Wei-Sen; Jia, Chun-Hong; Wei, Xiao-Xia; Feng, Ju-Ling; Zhao, Li; Wang, Li-Sheng

doi:10.1016/j.arcmed.2010.05.002

« Previous Next »Archives of Medical Research
Volume 41, Issue 4 , Pages 288-294, May 2010

Anti-oxidant Effects of Resveratrol on Mice with DSS-induced Ulcerative Colitis

Jun Yao
- Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China
Jian-Yao Wang
- Department of General Surgery, Shenzhen Municipal People's Hospital, Jinan University of Medical Sciences, Shenzhen, Guangdong Province, China
Lei Liu
- Department of General Surgery, Shenzhen Municipal People's Hospital, Jinan University of Medical Sciences, Shenzhen, Guangdong Province, China
Ying-Xue Li
- Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China
An-Ying Xun
- Department of Gastroenterology, Peking University Shenzhen Hospital, Shenzhen, Guangdong Province, China
Wei-Sen Zeng
- Department of Cell Biology, Southern Medical University, Guangzhou, Guangdong Province, China
Chun-Hong Jia
- Department of Cell Biology, Southern Medical University, Guangzhou, Guangdong Province, China
Xiao-Xia Wei
- Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China
Ju-Ling Feng
- Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China
Li Zhao
- Department of Cell Biology, Southern Medical University, Guangzhou, Guangdong Province, China
Li-Sheng Wang
- Department of Gastroenterology, Jinan University of Medical Sciences, Shenzhen Municipal People's Hospital, Shenzhen, Guangdong Province, China
- Address reprint requests to: Li-Sheng Wang, Department of Gastroenterology, Shenzhen Municipal People's Hospital, Jinan University of Medical Sciences, 1017 East Gate Road, Shenzhen, 518020, Guangdong Province, China; Phone: 0086-755-25533018; FAX: 0086-755-25533497

Received 1 November 2009; accepted 27 April 2010.

(ARCMED-D-09-00538)

Background and Aims

Oxidant/antioxidant balance is suggested to be an important factor for the recurrence and progression of ulcerative colitis (UC). The aim of the study is to investigate the potential protective role of resveratrol (Res) against dextran sodium sulfate (DSS)-induced oxidative damage in colon of mice with UC.

Methods

UC was induced in mice by oral administration of synthetic DSS (molecular weight 5000) for 7 days. Mice were divided into normal group, colitis control group, low-dose Res-treated group (RLD-treated group), and high-dose Res-treated group (RHD-treated group). Inhibitory effects of concomitant treatment with Res were assessed daily using a Disease Activity Index (DAI) and severity of histological changes. MDA, MPO, SOD and GSH-PX activity of colonic tissue were determined in colon samples by chemical colorimetry. TNF-α, IL-8, IFN-γ, p22^phox and gp91^phox expression levels were detected using quantitative reverse-transcriptase polymerase chain reaction (qRT-PCR), ELISA, and Western blot analysis.

Result

Administration of Res significantly inhibited the severity of UC compared to the colitis control group. Colonic tissue MDA and MPO activities decreased significantly in Res-treated groups compared to colitis control groups. Furthermore, colonic tissue SOD and GSH-Px activities increased significantly in Res-treated groups compared to colitis control groups. The expression levels of TNF-α, IL-8, IFN-γ, p22^phox, and gp91^phox also decreased significantly in the Res-treated group compared to the colitis control group.

Conclusions

Oral administration of Res exerts marked inhibitory effects on UC in mice. Resveratrol may play an important role in preventing DSS-induced oxidative damage.

Key Words: Resveratrol, Oxidative damage, Cytokines, Ulcerative colitis