Fetal Malnutrition Affects Hypothalamic Leptin Receptor Expression After Birth in Male Mice

Background and Aims

Epidemiological associations between an adverse intrauterine environment and the induction of obesity in adult life led to the concept of fetal programming whereby an unfavorable prenatal environment induces adaptations that improve fetal survival or prepare the fetus in expectation of a particular range of postnatal environments. However, these adaptations (predictive adaptive responses) may later prove to be a disadvantage when the pre- and postnatal environments show discrepancies. We investigated the effect of maternal restricted diet on body weight and expression of hypothalamic Ob-Rb of the offspring.

Methods

Balb C mice were mated after pregnancy and were randomly assigned to control (C) and undernutrition group (UN) groups. Control group was allowed food ad libitum and UN group had a 50% restriction of food intake during gestation. In the present study we assessed changes in hypothalamic Ob-Rb mRNA by RT-PCR in offspring from C and UN groups.

Results

The offspring of UN at birth showed 17% less body weight compared with C, but at 90 days the UN had a greater body weight than C (p <0.01). The UN group also presented an increase in the expression of Ob-Rb at 90 postnatal days (p <0.01).

Conclusions

The results suggest that maternal caloric restriction programs a greater expression of Ob-Rb in the hypothalamus in offspring, as well as a body weight gain that persists into adulthood. In addition, changes in Ob-Rb expression suggest that Ob-Rb mRNA in the hypothalamus is sensitive to fetal undernutrition.

Key Words: Leptin, Hypothalamus, Receptor Ob-Rb, Fetal malnutrition